TY - JOUR
AU - Glazko, Anthony, J
AB - Abstract Diphenylhydantoin (DPH) is extracted from plasma with ethylene dichloride, returned to alkali, and oxidized with permanganate in glass-stoppered test tubes containing a layer of iso-octane. The absorbance of the oxidation product (benzophenone) in the iso-octane layer is measured spectrophotometrically. This procedure eliminates interference by chloroform that is encountered in other methods; highly specialized reflux condensers or distillation equipment is not needed; and the cost of solvents is decreased. The procedure is highly specific; it detects less than 1 µg of DPH per milliliter of plasma, and it is suitable for routine clinical laboratory use. 1,2-dichloroethane as extractant This content is only available as a PDF. © 1971 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Simplified Benzophenone Procedure for Determination of Diphenylhydantoin in Plasma
JF - Clinical Chemistry
DO - 10.1093/clinchem/17.12.1200
DA - 1971-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/simplified-benzophenone-procedure-for-determination-of-FXc08mf11q
SP - 1200
EP - 1201
VL - 17
IS - 12
DP - DeepDyve
ER -