TY - JOUR
AU - Terao, Toshihiko
AB - Objective: Endometriosis, a common disease among women of reproductive age, is characterized by the presence of endometrial-like tissue outside the uterus. TNF-α induces IL-8 production in endometriotic cells through nuclear factor-κB (NF-κB) activation. Thalidomide (Thal) inhibits inflammation by down-regulating the expression of proinflammatory cytokines in tumor cells and inflammatory cells. However, the mechanism of Thal action in human endometriotic stromal cells has not yet been elucidated.Main Outcome Measures: We examined whether Thal abrogates TNF-α-induced up-regulation of IL-8 expression in endometriotic stromal cells.Results: Here, we show 1) that treatment of endometriotic stromal cells with TNF-α increased the expression of phosphorylated IκBα and degradation of total IκBα, which in turn activates NF-κB; 2) Thal significantly inhibits the TNF-α-induced expression of phosphorylated IκBα and degradation of IκBα; 3) TNF-α activation induced increased nuclear translocation of NF-κB, which was inhibited by pretreatment with either Thal or N-tosyl-l-phenylalanine chloromethyl ketone, an NF-κB inhibitor. Thal did not enhance the N-tosyl-l-phenylalanine chloromethyl ketone’s action; and 4) Pretreatment with Thal reduced TNF-α-induced IL-8 protein production as well as mRNA expression.Conclusion: The current study showed for the first time that Thal treatment attenuated the expression of IL-8 by reducing TNF-α-induced NF-κB activation.
TI - Thalidomide Inhibits Tumor Necrosis Factor-α-Induced Interleukin-8 Expression in Endometriotic Stromal Cells, Possibly through Suppression of Nuclear Factor-κB Activation
JF - Journal of Clinical Endocrinology and Metabolism
DO - 10.1210/jc.2004-1946
DA - 2005-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/thalidomide-inhibits-tumor-necrosis-factor-induced-interleukin-8-FRt46G4n2v
SP - 3017
EP - 3021
VL - 90
IS - 5
DP - DeepDyve
ER -