TY - JOUR
AU - Schluederberg, Ann
AB - Structures with the dimension and morphology of Dane particle cores were isolated and concentrated by a simple one-step procedure from human serum containing hepatitis B antigen. The method involves centrifugation of serum layered over a density gradient containing a zone of Nonidet-P40. Cores found at a solution density of 1.29–1.30 g/ml in Ficoll-sucrose gradients prepared in D2O reacted, in immunodiffusion tests, with a human serum containing core antibody. A modification of the method yielded partially degraded Dane particles in a highly aggregated array that revealed a previously undescribed outer morphology. Cores were surrounded by an areola containing regularly spaced projections 14 nm long that appeared to originate at individual sites on the surface of the core.
TI - Isolation of Cores from Hepatitis B Dane Particles
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/128.5.664
DA - 1973-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/isolation-of-cores-from-hepatitis-b-dane-particles-FGJEtO0CdA
SP - 664
EP - 667
VL - 128
IS - 5
DP - DeepDyve
ER -