TY - JOUR
AU - Nakai,, Masuyo
AB - Abstract We describe a procedure for in situ hybridization using a biotinylated Epstein-Barr virus (EBV) sequence with detection at the light and electron microscopic levels. In situ hybridization using an immunogold-silver staining detection system was used to identify biotinylated DNA probes in cell smears and in Lowicryl K4M-embedded EBV-infected and -noninfected cell lines. At the light microscopic level, the reaction product of hybridized EBV DNA sequence seemed to be located mainly in the nuclei. The labelling was dependant on the cell strains. However, at the electron microscopic level, the reaction product was evident as spots or dusters distributed not only in the nuclei of EBV-infected cells but also in the cytoplasm and extracellular particles. These findings suggest that immature particles in the cytoplasm contain EBV DNA. This procedure can be applied to the observation and identification of virus infection. electron microscopic in situ hybridization, Epstein-Barr virus (EBV), goldlabelling, biotinylated DNA probe, ultrathin section This content is only available as a PDF. © Japanese Society of Electron Microscopy
TI - Detection of Epstein–Barr virus DNA in virus-infected cells by electron microscopic in situ hybridization
JF - Journal of Electron Microscopy
DO - 10.1093/oxfordjournals.jmicro.a023539
DA - 1997-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/detection-of-epstein-barr-virus-dna-in-virus-infected-cells-by-Ec13dY9pvP
SP - 431
EP - 437
VL - 46
IS - 5
DP - DeepDyve
ER -