TY - JOUR
AU - 
AB - <jats:title>Abstract</jats:title>
               <jats:sec>
                  <jats:title>Background</jats:title>
                  <jats:p>CD8+ T cells play important roles in the antiviral immune response to HIV-1 infection. Variation in the antiviral activities of CD8+ T cells among HIV-1-infected persons has been associated with disease state and clinical prognosis. However, the anti-HIV activities of CD8+ T cells from healthy uninfected blood donors have not been fully established.</jats:p>
               </jats:sec>
               <jats:sec>
                  <jats:title>Methods</jats:title>
                  <jats:p>In this study we assessed the ability of peripheral blood CD8+ T cells from healthy blood bank donors to inhibit HIV-1-replication. Co-culture assays were performed with CD8+ T cells and acutely HIV-1-infected primary CD4+ T cells. Also, CD8+ T cells were cultured and the derivative supernatants were evaluated in virus inhibition assays, ELISAs, and multiplex cytokine arrays.</jats:p>
               </jats:sec>
               <jats:sec>
                  <jats:title>Results</jats:title>
                  <jats:p>Variation was observed among CD8+ T cells from different blood donors in the ability to suppress HIV-1 replication. In the absence of stimulation, the CD8+ T cells, or their derivative fluids, lacked any appreciable anti-HIV activity. After PHA or anti-CD3/CD28 stimulation, the majority of CD8+ T cells and derivative fluids exhibited anti-HIV activity. Low levels of interferon-alpha were present in the CD8+ T cell fluids having increased anti-HIV activity, whereas CD8+ T cell fluids lacking anti-HIV activity contained undetectable levels of interferon-alpha. Other soluble factors present at increased levels in CD8+ T cell fluids having anti-HIV activity included TNF-beta, IL-8, IP-10, and TRAIL.</jats:p>
               </jats:sec>
               <jats:sec>
                  <jats:title>Conclusions</jats:title>
                  <jats:p>Our findings indicate that the secretion of antiviral levels of interferon-alpha is a normal function of CD8+ T cells in response to activation and stimulation. Variation in this immune response may influence host susceptibility to infection and disease.</jats:p>
               </jats:sec>
TI - CD8+ T cells from healthy blood bank donors secrete antiviral levels of interferon-alpha
JF - The Journal of Immunology
DO - 10.4049/jimmunol.198.supp.125.6
DA - 2017-05-01
UR - https://www.deepdyve.com/lp/crossref/cd8-t-cells-from-healthy-blood-bank-donors-secrete-antiviral-levels-of-DO01tEg3NX
SP - 125.6
EP - 125.6
VL - 198
IS - 1_Supplement
DP - DeepDyve
ER -