TY - JOUR
AU - Sakamoto,, Yutaka
AB - Abstract Effects of various nitrogen-containing compounds on gametic differentiation and dedifferentiation in Chlamydomonas reinhardtii were studied. Vegetative cells grown either non-synchronously or synchronously differentiated to gametes within 8 h in NH4Cl-free medium. Addition of arginine did not interfere with gametic differentiation although arginine was utilized by the gametes for the progression of the cell cycle. Fully differentiated gametes dedifferentiated rapidly to vegetative cells upon feeding with NH4+ ions but not with arginine. Other utilizable sources of organic nitrogen, such as glutamine, ornithine, and argininosuccinate, were also unable to induce the dedifferentiation of gametes. When gametes were incubated with DL-methionine-DL-sulfoximine (MSX) and NH4+ ions, the assimilation of NH4+ ions appeared to be blocked by MSX, as indicated by the cessation of growth, but the cells rapidly lost their mating ability. These results indicate that the NH4+ ion itself, and not some metabolite, controls gametic differentiation and dedifferentiation in C. reinhardtii. This content is only available as a PDF. © 1992. The Japanese Society of Plant Physiologists (JSPP)
TI - Ammonium Ions Control Gametic Differentiation and Dedifferentiation in Chlamydomonas reinhardtii
JF - Plant and Cell Physiology
DO - 10.1093/oxfordjournals.pcp.a078340
DA - 1992-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/ammonium-ions-control-gametic-differentiation-and-dedifferentiation-in-D36eu9z3Uf
SP - 909
EP - 914
VL - 33
IS - 7
DP - DeepDyve
ER -