TY - JOUR AU - THIEL, S AB - SUMMARY The concentrations of mannan-binding protein (MBP) in consecutive samples from 10 HIV+ persons were estimated using an ELISA based on polyclonal rabbit anti-MBP. The changes in MBP with time were similar in HIV+ and HIV− persons, and did not appear to be of clinical significance. MBP was determined in a further 70 persons found HIV-1+ during a period af 2·5 years (1984–1986). Out of the total of 80 patients, 32 have by now died from AIDS. According to the serum level of MBP the HIV-infected persons were grouped into high (> 650 ng MBP/ml). intermediate (101–650 ng/ml), and low MBP (< 101 ng/ml). At the termination of the study the frequency of deaths/total in each of the groups were: high MBP, 14/39 (36%); intermediate MBP, 12/26 (46%); and low MBP, 6/14 (43%). There was no association between the MBP level of the individual and the progressive loss of CD4+ T cells, and the level of MBP was not predictive for the length of time between the detection of HIV antibodies and development of AIDS, nor for the duration of AIDS before death occurred. The number of HIV+ persons without detectable MBP (10%) was significantly higher than previously reported for healthy persons (2·4%, P= 0·027). The course of HIV infection does not seem to be influenced by the level of MBP, nor does the antimicrobial activity of MBP appear to affect the progression of AIDS. Further studies are required to substantiate the significance of absence of MBP in the susceptibility to HIV. HIV-1, mannan-binding protein, mannose-binding protein, MBP, innate immune system References 1 Malhotra R , Thiel S, Jensenius JC, Sim RB. Interaction of lung surfactant protein A with C1q . Compl Inflam . 1991 ; 8 : 188 (Abstr.). Google Scholar OpenURL Placeholder Text WorldCat 2 Kawasaki N , Kawasaki T, Yamashina I. Isolation and characterization of a mannan-binding protein from human serum . J Biochem 1983 ; 94 : 937 – 47 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Drickamer K , Dordal MS, Reynolds L. Mannose-binding proteins isolated from rat liver contain carbohydrate-recognition domains linked to collagenous tails. Complete primary structure and homology with pulmonary surfactant apoprotein . J Biol Chem . 1986 ; 261 : 6878 – 87 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Weis WI , Kahn R, Fourme R, Drickamer K, Hendrickson WA. Structure of the calcium-dependent lectin domain from a rat mannose-binding protein determined by MAD phasing . Science 1991 ; 254 : 1608 – 15 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Ikeda K , Sannoh T, Kawasaki N, Kawasaki T, Yamashina I. Serum lectin with known structure activates complement through the classical pathway . J Biol Chem 1987 ; 262 : 7451 – 554 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Lu JH , Thiel S, Wiedemann H, Timpl R, Reid KB. Binding of the pentamer/hexamer forms of mannan-binding protein to zymosan activates the proenzyme C1r2C1s2 complex of the classical pathway of complement without involvement of C1q . J Immunol 1990 ; 144 : 2287 – 94 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 7 Kuhlman M , Joiner K, Ezekowitz AB. The human mannose-binding protein function as an opsonin . J Exp Med 1989 ; 169 : 1733 – 45 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Malhotra R , Thiel S, Reid KB, Sim RB. Human leukocyte C1q receptor binds other soluble proteins with collagen domains . J Exp Med 1990 ; 172 : 955 – 9 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Super M , Thiel S, Lu J, Levinsky RJ, Turner MW. Association of low levels of mannan-binding protein with a common defect of opsonisation . Lancet 1989 ; ii : 1236 – 9 . Google Scholar OpenURL Placeholder Text WorldCat 10 Garred P , Thiel S, Madsen HO, Ryder LP, Jensenius JC, Svejgaard A. Gene frequency and partial protein characterization of an allelic variant of mannan-binding protein associated with low serum concentrations . Clin Exp Immunol 1992 ; 90 : 517 – 21 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Sumiya M , Super M, Tabona P, Levinsky RJ, Arai M, Turner MW. Summerfield JA, Molecular basis of opsonic defect in immunodeficient children . Lancet 1991 ; 337 : 1569 – 70 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Madsen HO , Garred P, Kurtzhals JA, Lamm LU, Ryder LP, Thiet S, Svejgaard A. A new prequent allele is the missing link in the structural polymorphism of the human mannan-binding protein . Immunogenet 1994 ; 40 : 37 – 44 . Google Scholar Crossref Search ADS WorldCat 13 Super M , Levinsky RJ, Turner MW. The level of mannan-binding protein regulates the binding of complement-derived opsonins to mannan and zymosan at low serum concentrations . Clin Exp Immunol 1990 ; 79 : 144 – 50 . Google Scholar Crossref Search ADS PubMed WorldCat 14 Thiel S , Holmskov U, Hviid L, Laursen SB, Jensenius JC. The concentration of the C-type lectin mannan-binding protein in human plasma increases during an acute phase response . Clin Exp Immunol 1992 ; 90 : 31 – 35 . Google Scholar Crossref Search ADS PubMed WorldCat 15 Ezekowitz RA , Kuhlman M, Groopman JE, Byrn RA. A human serum mannose-binding protein inhibits in vitro infection by the human immunodeficiency virus . J Exp Med 1989 ; 169 : 185 – 96 . Google Scholar Crossref Search ADS PubMed WorldCat 16 Haurum J , Thiel S, Jones IM, Fischer PB, Laursen SB, Jensenius JC. Complement activation upon binding of mannan-binding protein to HIV envelope glycoproteins . AIDS 1993 ; 7 : 1307 – 13 . Google Scholar Crossref Search ADS PubMed WorldCat 17 Sölder BM , Schulz TF, Hengster Pt et al. HIV and HIV-infected cells differentially activate the human complement system independent of antibody . Immunol Lett 1989 ; 22 : 135 – 45 . Google Scholar Crossref Search ADS PubMed WorldCat 18 Robinson WE , Montefiori DC, Mitchell WM. Antibody-dependent enhancement of human immunodeficiency virus type 1 infection . Lancet 1988 ; i : 790 – 4 . Google Scholar OpenURL Placeholder Text WorldCat 19 Robinson WE , Montefiori DC, Mitchell WM. Complement-mediated antibody-dependent enhancement of HIV-1 infection requires CD4 and complement receptors . Virology 1990 ; 175 : 600 – 4 . Google Scholar Crossref Search ADS PubMed WorldCat 20 Reisinger ED , Vogetseder W, Berzow D et al. Complement-mediated enhancement of HIV-1 infection of the monoblastoid cell line U937 . AIDS 1990 ; 4 : 961 – 5 . Google Scholar Crossref Search ADS PubMed WorldCat 21 Boyer V , Desgranges C, Trabaud MA, Fischer E, Kazatchkine MD. Complement mediates human immunodeficiency virus type 1 Infection of a human T cell line in a CD4- and antibody-independent fashion . J Exp Med 1991 ; 173 : 1151 – 8 . Google Scholar Crossref Search ADS PubMed WorldCat 22 Spear GT , Sulivan BL, Landay AI, Lint TF. Neutralization of human immunodeficiency virus type 1 by complement occurs by viral lysis . J Virol 1990 ; 64 : 5869 – 73 . Google Scholar Crossref Search ADS PubMed WorldCat 23 Perricone R , Fontana L, de Carolis C, Carini C, Sirianni MC, Aiuti F. Evidence for activation of complement in patients with AIDS related complex (ARC) and/or lymphoadenopathy syndrome (LAS) . Clin Exp Immunol 1987 ; 70 : 500 – 7 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 24 Senaldi G , Peakman M, Mcmanus T, Davies ET, Tee DE, Vergani D. Activation of the complement system in human immunodeficiency virus infection: relevance of the classical pathway to pathogenesis and disease severity . J Infect Dis 1990 ; 162 : 1227 – 32 . Google Scholar Crossref Search ADS PubMed WorldCat 25 Centers for Disease Control . Revision of the CDC surveillance case definition for acquired immunodeficience syndrome . MMWR 1987 ; 36 ( suppl. 1S ): 1 – 15 . OpenURL Placeholder Text WorldCat 26 Horowitz B , Wiebe ME, Lippin A, Stryker MH. Inactivation of viruses in labile blood derivatives. Disruption of lipid enveloped viruses by tri-(n-buty)-phosphate detergent combinations . Transfusion . 1985 ; 25 : 516 – 22 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) TI - The level of the serum opsonin, mannan-binding protein in HIV-1 antibody-positive patients JF - Clinical & Experimental Immunology DO - 10.1111/j.1365-2249.1995.tb03656.x DA - 2008-06-28 UR - https://www.deepdyve.com/lp/oxford-university-press/the-level-of-the-serum-opsonin-mannan-binding-protein-in-hiv-1-D0JB0mS0sD SP - 219 EP - 222 VL - 100 IS - 2 DP - DeepDyve ER -