TY - JOUR
AU - ISHII,, FUMIE
AB - Abstract 1. The interaction between bovine pancreatic ribonuclease [EC 2.7.7.16] (RNase) and 4-thiouridine 2'(3')-phosphate was compared with that between RNase and uridine 2'(3')-phosphate. 2. The difference spectrum of the RNase-4-thiouridylic acid complex was similar in shape to that of the RNase-uridylic acid complex, but was at about 70 μ longer wavelengths. 3. The pH dependence of the spectral change (hypochromism) of the two complexes were similar, the maxima being at between pH 5 and 6. 4. The dissociation constants of the both complexes were estimated by spectrophotometric titration to be 1.1×10−5m at pH5.6, 0.05 ionic strength and 25°G. 5. Gel filtration showed that both nucleotides bind to the enzyme in a one to one ratio. 6. These results indicate that the modes of interaction of the two nucleotides with RNase are similar. This content is only available as a PDF. Author notes *On leave from Kitasato University, Tokyo © 1968 by the Journal of Biochemistry
TI - Interaction between Bovine Pancreatic Ribonuclease and 4-Thiouridylic Acid
JF - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a128875
DA - 1968-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/interaction-between-bovine-pancreatic-ribonuclease-and-4-thiouridylic-Cw3XQ3x0gN
SP - 161
EP - 165
VL - 64
IS - 2
DP - DeepDyve
ER -