TY - JOUR AU - AB - We used random amplification of polymorphic DNA (RAPD) to generate species-specific diagnostic fragment pat- terns for the molecular identification of the ornamental aquarium fish species Badis badis and Dario dario. Seven ar- bitrary oligodecamer primers produced a total of 116 bands of which 98.23% were polymorphic. The size of the amplified products was in the range 340 bp to 2170 bp. Intraspecies genetic similarity was 0.879 ± 0.023 for B. badis and 0.840 ± 0.014 for D. dario while interspecies genetic similarity was 0.602 ± 0.017, with cluster analysis displaying separate taxonomic and evolutionary status for these fish. The results show that RAPD was useful for the molecular identification of aquarium fish species, with morphological traits also being important. Key words: Badis badis, Dario dario, diagnostic markers, RAPD molecular identification, West Bengal. Received: July 11, 2007; Accepted: November 26, 2007. India is endowed with a vast fish biological resource and Britz (2002) revised the family Badidae and placed representing more than 10% of world fish diversity (Das Badis badis bengalensis in the new genus Dario. and Pandey, 1998). However, most indigenous aquarium The objective of the study described in this paper was fish have not been genetically characterized and TI - RAPD fingerprinting of the ornamental fish Badis badis (Hamilton 1822) and Dario dario (Kullander and Britz 2002) (Perciformes, Badidae) from West Bengal, India JO - Genetics and Molecular Biology DO - 10.1590/s1415-47572008000400028 DA - 2008-01-01 UR - https://www.deepdyve.com/lp/unpaywall/rapd-fingerprinting-of-the-ornamental-fish-badis-badis-hamilton-1822-CqwD3613SM DP - DeepDyve ER -