TY - JOUR
AU - Nagataki,, S.
AB - Abstract We have previously demonstrated that interferon-y (IFN-Y) induced HLA-DR antigen and also inhibited thyrotropin (TSH)-induced triiodothyronine (T3) and thyroglobulin (Tg) secretion from cultured human thyrocytes. In order to further clarify the inhibitory effect of IFN-y on TSH-stimulated thyroid hormone secretion, we have examined human thyroid peroxidase (TPO∧ gene expression. Thyrocytes dispersed from Graves' thyroid tissues were incubated with TSH with or without IFN-y. Total RNA was extracted, separated and hybridized with 32P-labelled human TPO cDNA. Thyrocytes expressed four TPO mRNA transcripts (4.0, 3.2, 2.1 and 1.7kb, respectively), all of which were stimulated by TSH. IFN-Y inhibited TSH-stimulated TPO mRNA in a dose dependent nanner (0.01-10U/mL). 1 U/mL IFN-y caused maximal suppression of TSH-stimulated TPO mRNA levels to basal levels. IFN-Y also inhibited 8-bromo-cyclic AMP-stimulated TPO mRNA levels. In contrast, the y-actin mRNA hybridization signal was not altered in control or treated cells. These results demonstrate that IFN-y directly inhibits TSH-stimulated TPO gene expression and provide further support for a role of IFN-Y as a local modulator of thyroid hormone synthesis. This content is only available as a PDF. Author notes * To whom correspondance and reprint requests should be addressed. Copyright © 1989 by The Endocrine Society
TI - INTERFERON-y INHIBITS THYROTROPIN-INDUCED THYROIDAL PEROXIDASE GENE EXPRESSION IN CULTURED HUMAN THYROCYTES
JF - Journal of Clinical Endocrinology and Metabolism
DO - 10.1210/jcem-69-2-475
DA - 1989-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/interferon-y-inhibits-thyrotropin-induced-thyroidal-peroxidase-gene-CmISauMwer
SP - 475
EP - 477
VL - 69
IS - 2
DP - DeepDyve
ER -