TY - JOUR AU - Pedrós-Alió, Carlos AB - AbstractThe diversity of prokaryotic and eukaryotic phytoplankton was studied along a gradient of salinity in the solar salterns of Bras del Port in Santa Pola (Alacant, Spain) using different community descriptors. Chlorophyll a, HPLC pigment composition, flow cytometrically-determined picoplankton concentration, taxonomic composition of phytoplankton (based on optical microscopy) and genetic fingerprint patterns of 16S (cyanobacteria- and chloroplast-specific primers) and 18S rRNA genes were determined for samples from ponds with salinities ranging from 4% to 37%. Both morphological and genetical descriptors of taxonomic composition showed a good agreement and indicated a major discontinuity at salinities between 15% and 22%. The number of classes and the Shannon diversity index corresponding to the different descriptors showed a consistent decreasing trend with increasing salinity. The results indicate a selective effect of extremely high salinities on phytoplanktonic assemblages. TI - Diversity of planktonic photoautotrophic microorganisms along a salinity gradient as depicted by microscopy, flow cytometry, pigment analysis and DNA-based methods JF - FEMS Microbiology Ecology DO - 10.1016/j.femsec.2004.04.002 DA - 2004-08-05 UR - https://www.deepdyve.com/lp/oxford-university-press/diversity-of-planktonic-photoautotrophic-microorganisms-along-a-CLj4J4Pzrc SP - 281 EP - 293 VL - 49 IS - 2 DP - DeepDyve ER -