TY - JOUR
AU1 - Iwata, J.
AU2 - Oka, T.
AU3 - Furihata, M.
AU4 - Sonobe, H.
AU5 - Matsubayashi, K.
AU6 - Uemura, Y.
AU7 - Miyoshi, I.
AU8 - Ohtsuki, Y.
AB - Two cell lines, CNS-5 and CNS-6, were established by cocultivation of sedimented cells in cerebrospinal fluid (CSF) from two anti-human T-lymphotropic virus type I (HTLV-I) antibody-positive male patients with encephalopathy and HTLV-I-associated myelopathy, respectively, with peripheral blood mononuclear cells from a healthy seronegative female. These cell lines, possessing a normal female karyotype, revealed similar characteristics as follows; they expressed HTLV-I-related antigens, they produced C-type retrovirus particles, HTLV-I provirus genomes were integrated into their DNAs, and they had CD4+ activated T-cell markers. In addition, immunocytochemical and immunoelectron microscopic studies showed peculiar immunoreactivity of these cell lines with anti-α/β T-cell antigen receptor (TCR) antibodies; β F1, defining β chain epitope, was only positive in the perinuclear spaces and rough endoplasmic reticulum in some cells, and WT31, recognizing α/β framework, was mostly negative, while CD3 was expressed in the majority of the cells.
TI - Characterization of two human lymphoid cell lines producing human T-lymphotropic virus type I (HTLV-I) isolated from patients with HTLV-I-associated myelopathy or encephalopathy
JF - Archives of Virology
DO - 10.1007/BF01311306
DA - 2005-02-28
UR - https://www.deepdyve.com/lp/springer-journals/characterization-of-two-human-lymphoid-cell-lines-producing-human-t-CLMBJNCxWf
SP - 101
EP - 112
VL - 118
IS - 2
DP - DeepDyve
ER -