TY - JOUR
AU - Gascoyne, Norman
AB - The ganglioside galactosyl-N-acetylgalactosaminyl [sialosyl] lactosylceramide (GM1) is readily accumulated in pigeon red cell membranes soaked with [3H]GM1 (1–100 µg/ml) at 37 C for 30 min. This treatment enhances the activation of adenyl cyclase by the toxin of Vibrio cholerae. An attempt was made at correlation of the amount of incorporated GM1 with the increased binding of toxin and activation of adenyl cyclase. Cells with less than 2 µg of incorporated G~n per 4 × 109 cells bind 5–10 µg more toxin than do untreated cells, which bind 0.25 µg per 4 × 109 cells. Cells with more than 2 µg of GM1 bound (per 4 × 109 cells) which has been incorporated from micellar solutions of GMt (>20 µg/ml) , do not bind any more extra toxin. In untreated cells, 0.1 µg of toxin is involved in the activation of adenyl cyclase. In GM1-treated cells 0.25–0.5 fig of toxin is involved, although at least 5 fig of toxin is bound. It is concluded that 90% of the extra toxin-binding sites on the GM1-treated cell are nonproductive.
TI - Aspects of the Interaction of Vibrio cholerae Toxin with the Pigeon Red Cell Membrane
JO - The Journal of Infectious Diseases
DO - 10.1093/infdis/133.Supplement_1.S75
DA - 1976-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/aspects-of-the-interaction-of-vibrio-cholerae-toxin-with-the-pigeon-C8qc0YnWuF
SP - S75
EP - S81
VL - 133
IS - Supplement_1
DP - DeepDyve
ER -