TY - JOUR
AU1 - Yehouenou, Carine Laurence
AU2 - Kpangon, Arsène A.
AU3 - Affolabi, Dissou
AU4 - Rodriguez-Villalobos, Hector
AU5 - Van Bambeke, Françoise
AU6 - Dalleur, Olivia
AU7 - Simon, Anne
AB - BackgroundSurgical site infections are related to high morbidity, mortality and healthcare costs. Because the emergence of multidrug-resistant bacteria in hospitals is becoming a worldwide challenge for surgeons who treat healthcare-associated infections, we wished to identify the causative agents involved in these infections and the rate of multidrug-resistant bacteria in six public hospitals in Benin.MethodsUsing standard microbiological procedures, we processed pus specimens collected from obstetrics and gastrointestinal surgery wards. Mass spectrometry (MALDI-TOF) was used for confirmation. For the antibiotic susceptibility test, we first used the Kirby-Bauer disk diffusion method. The secondary test (by microdilution) used the Beckton Dickinson Phoenix automated system (Becton Dickinson Diagnostic, USA).ResultsWe included 304 patients, whose median length of stay was 9 days. A total of 259 wound swabs (85.2%) had positive aerobic bacterial growth. In obstetrics, S. aureus (28.5%, n = 42) was the most common isolate. In contrast, Gram-negative bacteria (GNB) were predominant in gastrointestinal surgery, the most dominant being E.coli (38.4%, n = 31). Overall, 90.8% (n = 208) of aerobic bacteria were multidrug resistant. Two-thirds of S. aureus (65.3%, n = 32) were methicillin-resistant Staphylococcus aureus (MRSA), three of which carried both MRSA and induced clindamycin resistance (ICR). GNB showed high resistance to ceftazidime, ceftriaxone and cefepime. Extended-spectrum beta-lactamases were presented by 69.4% of E.coli (n = 43/62) and 83.3% of K. pneumoniae (n = 25/30). Overall, twelve Gram-negative bacteria (5.24%) showed resistance to at least one carbapenem. No isolates showed a wild-type susceptible phenotype.ConclusionThis study shows the alarming prevalence of multidrug-resistant organisms from surgical site infections in Benin hospitals. To reduce the spread of such bacteria in Benin, periodic surveillance of surgical site infections and strict adherence to good hand-hygiene practice are essential.
TI - Antimicrobial resistance in hospitalized surgical patients: a silently emerging public health concern in Benin
JF - Annals of Clinical Microbiology and Antimicrobials
DO - 10.1186/s12941-020-00398-4
DA - 2020-12-01
UR - https://www.deepdyve.com/lp/springer-journals/antimicrobial-resistance-in-hospitalized-surgical-patients-a-silently-C0xTuwGeMd
VL - 19
IS - 1
DP - DeepDyve
ER -