TY - JOUR AU - Umezawa, Yoshio AB - Fluorescence imaging is a powerful technique to visualize spatiotemporal dynamics of biomolecules in living cells. We describe fluorescent indicators for a lipid second messenger, diacylglycerol (DAG), which allow the localized analysis of DAG dynamics at subcellular membranes. We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes. TI - Imaging diacylglycerol dynamics at organelle membranes JO - Nature Methods DO - 10.1038/nmeth930 DA - 2006-09-21 UR - https://www.deepdyve.com/lp/springer-journals/imaging-diacylglycerol-dynamics-at-organelle-membranes-BcHUlmHgo4 SP - 797 EP - 799 VL - 3 IS - 10 DP - DeepDyve ER -