TY - JOUR
AU - Smith, Lewis J.
AB - Among the toxic effects of hyperoxia may be impaired pulmonary clearance of gram-negative bacteria. To better define this effect, we exposed BALBIc mice to 100% O2 for 24, 48, or 72 hr and intrabronchially inoculated them with 106 Pseudomonas aeruginosa. Clearance was assessed 4 hr later by quantitative lung cultures of air- and O2-exposed mice. Clearance was first reduced at 48 and 72 hr in mice exposed to O2. To determine the mechanism responsible, we measured bronchoalveolar lavage neutrophil (PMN) counts and neutrophil chemotactic activity at 0, 2, and 4 hr after instillation of P. aeruginosa into mice first exposed to air or O2 for 48 hr. Air-exposed mice had more PMNs than did O2-exposed mice after challenge (13.3 ± 2.1 × 105 vs. 4.4 ± 0.6 × 105 ) . There was no difference in neutrophil chemotactic activity between air- and O2-exposed mice at any time, although chemotactic activity increased in both groups after challenge. Our data suggest that hyperoxia impairs pulmonary clearance of P. aeruginosa by decreasing the influx of PMNs and that this effect is not due to diminished chemotactic activity in bronchoalveolar lavage fluid.
TI - The Effects of Hyperoxia on Pulmonary Clearance of Pseudomonas aeruginosa
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/153.4.676
DA - 1986-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/the-effects-of-hyperoxia-on-pulmonary-clearance-of-pseudomonas-BaI0Z0OjGd
SP - 676
EP - 681
VL - 153
IS - 4
DP - DeepDyve
ER -