TY - JOUR
AU1 - Beránek, Martin
AU2 - Sirák, Igor
AU3 - Vošmik, Milan
AU4 - Petera, Jiří
AU5 - Drastíková, Monika
AU6 - Palička, Vladimír
AU7 - 
AB - ORIGINAL ARTICLE Carrier Molecules and Extraction of Circulating Tumor DNA for Next Generation Sequencing in Colorectal Cancer 1,2,* 3 3 3 1 1 Martin Beránek , Igor Sirák , Milan Vošmik , Jiří Petera , Monika Drastíková , Vladimír Palička Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Charles University, Faculty of Medicine in Hradec Králové, Czech Republic Department of Biochemical Sciences, Charles University, Faculty of Pharmacy in Hradec Králové, Czech Republic Department of Oncology, University Hospital Hradec Králové, Charles University, Faculty of Medicine in Hradec Králové, Czech Republic * Corresponding author: Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Sokol- ská 581, 500 05 Hradec Králové, Czech Republic; e-mail: beranek@lfhk.cuni.cz Summary: The aims of the study were: i) to compare circulating tumor DNA (ctDNA) yields obtained by different manual extraction procedures, ii) to evaluate the addition of various carrier molecules into the plasma to improve ctDNA extrac- tion recovery, and iii) to use next generation sequencing (NGS) technology to analyze KRAS, BRAF, and NRAS somatic mutations in ctDNA from patients with metastatic colorectal cancer. Venous blood was obtained from patients who suf- fered from metastatic colorectal carcinoma. For plasma ctDNA extraction, the following carriers 
TI - Carrier molecules and extraction of circulating tumor DNA for next generation sequencing in colorectal cancer
JF - Acta Medica (Hradec Kralove, Czech Republic)
DO - 10.14712/18059694.2016.54
DA - 2016-01-01
UR - https://www.deepdyve.com/lp/unpaywall/carrier-molecules-and-extraction-of-circulating-tumor-dna-for-next-AqsylLWvTZ
DP - DeepDyve
ER -