TY - JOUR
AU - Hancock, Dale D.
AB - Foods of bovine origin have been linked to human disease outbreaks caused by Escherichia coli O157:H7 and may be linked to the more common sporadic cases as well. In this study, E. coli O157:H7 from the bovine reservoir (22 isolates: 12 from dairy and 10 from beef breed cows)and from human patients (50 isolates from sporadic human infections) were compared using Shigalike toxin genotypes, plasmid profiles, and DNA restriction fragment length polymorphisms identified with a bacteriophage λ probe (λ-RFLP). Twenty-three λ-RFLPprofiles, 4 Shiga-like toxin genotypes, and 8 plasmid profiles were identified among the isolates tested. Together the typing methods distinguished 43 strains, of which 3 were isolated from both humans (5 isolates) and cattle (6 isolates; 5 from dairy herds). These data demonstrate the value of λ-RFLP as a means of strain identification for E. coli O157:H7.
TI - A Comparison of Human and Bovine Escherichia coli O157:H7 Isolates by Toxin Genotype, Plasmid Profile, and Bacteriophage λ-Restriction Fragment Length Polymorphism Profile
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/168.5.1300
DA - 1993-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/a-comparison-of-human-and-bovine-escherichia-coli-o157-h7-isolates-by-AlRruPlFrd
SP - 1300
EP - 1303
VL - 168
IS - 5
DP - DeepDyve
ER -