TY - JOUR
AU - Sugiyama, Shigeru
AB - We have developed a rolling circle amplification-procedure as a method to enhance signals of immunoassay (immuno-RCA) to detect one of the major food allergens, ovalbumin (OVA). Immuno-RCA in combination with a fluorescent dye and a circular DNA probe prepared by intramolecular ligation easily allowed the real-time detection of OVA, and could apparently detect signals from OVA with concentrations ranging from 10–12 to 10–7 g/mL. Therefore, sensitive and easily handled aspects of this method would contribute to an effective signal enhancement in immunoassay for food allergen detections.
TI - Rolling Circle Amplification for Signal Enhancement in Ovalbumin Detection
JF - Analytical Sciences
DO - 10.2116/analsci.25.1381
DA - 2009-12-01
UR - https://www.deepdyve.com/lp/springer-journals/rolling-circle-amplification-for-signal-enhancement-in-ovalbumin-AOYj6SFS8A
SP - 1381
EP - 1383
VL - 25
IS - 12
DP - DeepDyve
ER -