TY - JOUR AU - AB - Background PCNA-associated factor (KIAA0101/p15PAF) is a cell-cycle regulated oncoprotein that regulates DNA synthesis, maintenance of DNA methylation, and DNA-damage bypass, through the interaction with the human sliding clamp PCNA. KIAA0101 is overexpressed in various cancers, including hepatocellular carcinoma (HCC). However, it remains unknown whether KIAA0101 gene ampli cation occurs and causally correlates with the KIAA0101 overexpression in HCC. This question is relevant to the development of the optimal test(s) for KIAA0101 and the strategies to target KIAA0101 in HCC. Methods In this study, we validated KIAA0101 mRNA expression levels by quantitative real-time PCR in 40 pairs of snap-frozen HCC and matched-non-cancerous tissues; we then evaluated KIAA0101 gene copy numbers by droplet digital PCR (ddPCR) in 36 pairs of the tissues. Besides, KIAA0101 protein expression was detected by immunohistochemistry (IHC) in 81 pairs of formalin- xed paran-embedded (FFPE) tissues. We determined the correlation between KIAA0101 gene copy numbers and KIAA0101 RNA expression by Spearman correlation, and between KIAA0101 protein expression and other clinicopathological variables by Chi-square test. Results Our results con rmed KIAA0101 mRNA overexpression in HCC; KIAA0101 mRNA levels were signi cantly higher in HCC than in the matched-non-cancerous tissues (p < 0.0001). We found no ampli cation of KIAA0101 gene TI - PCNA-associated Factor (KIAA0101/p15PAF) over expression and Gene Copy Number Alterations in Hepatocellular Carcinoma Tissues DO - 10.21203/rs.3.rs-39782/v1 DA - 2020-07-10 UR - https://www.deepdyve.com/lp/unpaywall/pcna-associated-factor-kiaa0101-p15paf-over-expression-and-gene-copy-AN09IHEf3n DP - DeepDyve ER -