TY - JOUR
AU - Li, Shuang
AB - Ethanol was a major byproduct of 2,3-butanediol (2,3-BD) fermentation by Klebsiella oxytoca ME-UD-3. In order to achieve a high efficiency of 2,3-BD production, K. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the aldA gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. The carbon flux to 2,3-BD was enhanced by eliminating the byproducing ethanol and at the same time reducing the accumulation of another byproduct acetoin. At last, by fed-batch culturing of the mutant, the final 2,3-BD titer up to 130 g/l with the productivity of 1.63 g/l.h and the 2,3-BD yield relative to glucose of 0.48 g/g was obtained.
TI - Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene
JF - Applied Microbiology and Biotechnology
DO - 10.1007/s00253-009-2222-2
DA - 2009-09-16
UR - https://www.deepdyve.com/lp/springer-journals/engineering-klebsiella-oxytoca-for-efficient-2-3-butanediol-production-A8C6SH9ZtU
SP - 1751
EP - 1758
VL - 85
IS - 6
DP - DeepDyve
ER -