TY - JOUR
AU - Yang,  H. Y.
AB - We have established a technique for isolating, culturing and transforming tobacco zygotes. Zygotes were isolated by microdissection or enzymatic maceration from fertilized embryo sacs. Viable zygotes cocultured with mesophyll protoplasts underwent first division after 3 days of culture. Zygotes isolated by microdissection underwent a higher frequency of first division (61.2%) than those isolated by enzymatic maceration (30.5%). Globular embryos were formed only from microdissected zygotes, at a frequency of 8.7% after 1–2 weeks in culture. An efficient millicell device for the electroporation of DNA into zygotes was established. The electroporated zygotes divided in vitro at a frequency of 54.6% and developed into proembryos. Introduced GFP gene constructs showed transient expression in about 2.6% of the electroporated tobacco zygotes.
TI - Gene transfer into isolated and cultured tobacco zygotes by a specially designed device for electroporation
JF - Plant Cell Reports
DO - 10.1007/s002990000249
DA - 2000-12-01
UR - https://www.deepdyve.com/lp/springer-journals/gene-transfer-into-isolated-and-cultured-tobacco-zygotes-by-a-9iHBXimVwN
SP - 1184
EP - 1187
VL - 19
IS - 12
DP - DeepDyve
ER -