TY - JOUR
AU - Randall Armant,, D.
AB - Abstract To extend our previous finding that ethanol stimulates murine preimplantation development, we focused in the current study on the cavitation and expansion of the blastocyst. Ethanol stimulation of blastocyst cavitation and expansion was optimal at a concentration of 0.1% and required only a 5-min exposure. Because intracellular levels of calcium were transiently increased in the ethanol-exposed embryos, we determined the effect of directly increasing calcium on the rates of blastocyst cavitation and expansion. Treatment with the calcium ionophore, A23187, altered development much as did ethanol, indicating that ethanol may stimulate preimplantation development by increasing the intracellular calcium concentration. The relationship between intracellular calcium levels and blastocoel volume suggests that morphogenetic events during preimplantation development, particularly cavitation and blastocyst formation, may be regulated through modulation of intracellular calcium levels. This content is only available as a PDF. Author notes 1 This work was supported by grants from the National Institutes of Health (HD25795 and P50AA07606–06) and Wayne State University (146641). JJ.S. was supported by an NIAAA Alcohol Research Training Grant AA07531–02. F.D.Y. was supported by a postdoctoral fellowship from the Lalor Foundation. Copyright © 1994 by The Society for the Study of Reproduction
TI - Blastocyst Cavitation is Accelerated by Ethanol- or Ionophore-Induced Elevation of Intracellular Calcium
JF - Biology of Reproduction
DO - 10.1095/biolreprod50.1.1
DA - 1994-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/blastocyst-cavitation-is-accelerated-by-ethanol-or-ionophore-induced-9Z3vr6QsYu
SP - 1
EP - 9
VL - 50
IS - 1
DP - DeepDyve
ER -