TY - JOUR
AU - Morad, Hamed
AB - Recurrent aphthous stomatitis (RAS) is one of the most common oral diseases which belongs to the category of inflammatory diseases. Although many studies have been conducted on the lesion, there is still no consensus on effective treatment of this lesion. Owing to the therapeutic effects of berberine, such as antibacterial, anti-inflammatory, and antioxidant effects, as well as the significant benefits of nanofibers (NFs) based on gelatin (Gel) and chitosan (CS), a new electrospun formulation has been fabricated in this study. This drug delivery system consists of a double-layer nanofibrous membrane of Gel-Cs active layer and a polylactic acid (PLA) inactive back. Morphological and physicochemical properties of NFs were characterized, and in vitro as well as in vivo study, including wound closure, histological and immune histochemical (IHC) analyses were performed for local drug delivery to heal aphthous ulcers. Based on the results of this study, at the optimum concentration of the solution (Gel75-CS25), smooth and bead-free NFs with an average diameter of 262 ± 82 nm were obtained. The infrared and X-ray spectra confirmed the berberine entrapment, proper interaction of the polymer-drug, and the lack of interaction of the polymer mixture with PLA. The comparison of the release behavior and the water contact angle indicated a controlled release of the mixed formulation. However, adding PLA coating reduced the release rate, increased the water contact angle, and improved the mechanical properties of the system. Finally, histological studies confirmed the effectiveness of the drug-containing system. The results indicated that this system can improve localized delivery, release kinetics, and healing without the adverse effects and limited efficacy often associated with existing treatments.Graphical Abstract[graphic not available: see fulltext]
TI - Berberine Encapsulated in Double-Layered Electrospun Nanofibrous Membrane for Recurrent Oral Aphthous Treatment: Fabrication, In Vitro Characterization, and In Vivo Studies
JO - BioNanoScience
DO - 10.1007/s12668-025-01850-5
DA - 2025-06-01
UR - https://www.deepdyve.com/lp/springer-journals/berberine-encapsulated-in-double-layered-electrospun-nanofibrous-901IocRYvh
VL - 15
IS - 2
DP - DeepDyve
ER -