TY - JOUR
AU - Hendry, I.A.
AB - Background Neurons require contact with their target tissue in order to survive and make correct connections. The retrograde axonal transport of neurotrophins occurs after receptor‐mediated endocytosis into vesicles at the nerve terminal. However, the mechanism by which the neurotrophin signal is propagated from axon terminal to cell body remains unclear. Methods Retrograde axonal transport was examined using the transport of I125‐labeled neurotrophins from the eye to sympathetic and sensory ganglia. The phenomena was further studied by adding rhodamine‐labeled nerve growth factor (NGF) to cultures of dissociated sympathetic ganglia and the movement of organelles followed with the aid of video microscopy. Results I125‐labeled neurotrophins were transported from the eye to the sympathetic and sensory ganglia. A 100‐fold excess of unlabeled neurotrophin, administered up to 4 h after the labeled material, completely prevented accumulation of labeled neurotrophin in the ganglia. The effect was specific for the labeled neurotrophin as administration of a high concentration of a different neurotrophin failed to inhibit the transport. In dissociated cultures, we found rapid binding of label, to surface membrane receptors, followed by an accumulation of labeled vesicles in the growth cone. Incubation of these cultures with unlabeled NGF led to a rapid loss of label in the growth cones. Conclusions These results suggest that there is a pool of internalized neurotrophin, in vesicles in the nerve terminal, which is in rapid equilibrium with the external environment. It is from this pool that a small fraction of the neurotrophin‐containing vesicles is targeted for retrograde transport. Potential models for this system are presented. Cytometry 43:182–188, 2001. © 2001 Wiley‐Liss, Inc.
TI - Prolonged recycling of internalized neurotrophins in the nerve terminal
JO - Cytometry
DO - 10.1002/1097-0320(20010301)43:3<182::AID-CYTO1047>3.3.CO;2-A
DA - 2001-03-01
UR - https://www.deepdyve.com/lp/wiley/prolonged-recycling-of-internalized-neurotrophins-in-the-nerve-8rXZO0PRPW
SP - 182
EP - 188
VL - 43
IS - 3
DP - DeepDyve
ER -