TY - JOUR
AU1 - EVERETT, N., P.
AB - Abstract 2,4-D-independent (ID) cell lines of Acer pseudoplatanus L. were isolated from a 2,4-D-dependent suspension culture at frequencies of about 3 × 10−6. This low frequency of occurrence, together with stable physiological differences between individual cell lines, suggested that loss of 2,4-D-dependence was the result of genetic, rather than epigenetic, change. Although some of the ID clones could be induced to become 2,4-D-responsive by a short treatment with 2,4-D, no reversions to 2,4-D-dependence were observed. When grown in the absence of 2,4-D, some ID clones were kinetin-dependent while others were kinetin-independent. Tests for resistance to 5-bromodeoxyuridine, 5-methyltryptophan, and methotrexate suggested that changes in the metabolism of thymidine, tryptophan, or folic acid are not prerequisites for either auxin- or cytokinin-independence. This content is only available as a PDF. Author notes 1Present address: Biochemistry Department, University of Liverpool, P.O. Box 147, Liverpool L69 3BX. © Oxford University Press
TI - 2,4-D-Independent Cell Cultures of Sycamore (Acerpseudoplatanus L.): Isolation, Responses to 2,4-D and Kinetin, and Sensitivities to Antimetabolites
JF - Journal of Experimental Botany
DO - 10.1093/jxb/32.1.171
DA - 1981-02-01
UR - https://www.deepdyve.com/lp/oxford-university-press/2-4-d-independent-cell-cultures-of-sycamore-acerpseudoplatanus-l-8qAwSo0xFn
SP - 171
EP - 182
VL - 32
IS - 1
DP - DeepDyve
ER -