TY - JOUR
AU - PHILLIPS, G. O.
AB - Initial investigations showed that certain populations of Sytyphimurium cells could be resolved into three fractions by eluting the cells from DEAE‐cellulose with a NaCl gradient in 0·05 Mphosphate buffer (pH 6·0). The three fractions were eluted with 0, 60 and 190 mmol/l NaCl. The results obtained with two strains indicate that non‐fimbriate, exponential phase cells; fimbriate, stationary phase cells; and non‐fimbriate, stationary phase cells are eluted with 0, 60 and 190 mmol/l NaCl in 0·05 M‐phosphate buffer (pH 6·0), respectively. These conclusions appear to be consistent with current views on the effects of fimbriae and the exponential growth phase in reducing the electrical charge carried by bacterial cells.
TI - Influence of the Growth Phase and Fimbriae on the Gradient Elution of Salmonella typhimurium from DEAE‐cellulose
JO - Journal of Applied Microbiology
DO - 10.1111/j.1365-2672.1978.tb00793.x
DA - 1978-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/influence-of-the-growth-phase-and-fimbriae-on-the-gradient-elution-of-7QR8wV4WI2
SP - 215
EP - 223
VL - 44
IS - 2
DP - DeepDyve
ER -