TY - JOUR
AU - Pelechano, Vicent
AB - Fast and accurate detection of nucleic acids is key for pathogen identification. Methods for DNA detection generally rely on fluorescent or colorimetric readout. The development of label-free assays decreases costs and test complexity. We present a novel method combining a one-pot isothermal generation of DNA nanoballs with their detection by electrical impedance. We modified loop-mediated isothermal amplification by using compaction oligonucleotides that self-assemble the amplified target into nanoballs. Next, we use capillary-driven flow to passively pass these nanoballs through a microfluidic impedance cytometer, thus enabling a fully compact system with no moving parts. The movement of individual nanoballs is detected by a change in impedance providing a quantized readout. This approach is flexible for the detection of DNA/RNA of numerous targets (severe acute respiratory syndrome coronavirus 2, HIV, β-lactamase gene, etc.), and we anticipate that its integration into a standalone device would provide an inexpensive (<$5), sensitive (10 target copies), and rapid test (<1 hour).
TI - Digital assay for rapid electronic quantification of clinical pathogens using DNA nanoballs
JF - Science Advances
DO - 10.1126/sciadv.adi4997
DA - 2023-09-06
UR - https://www.deepdyve.com/lp/pubmed-central/digital-assay-for-rapid-electronic-quantification-of-clinical-6NbIUJkC8W
VL - 9
IS - 36
DP - DeepDyve
ER -