TY - JOUR
AU - CAPLIN, SAMUEL M.
AB - Abstract WE HAVE employed with success, as a medium for the mounting and clearing of biologic specimens, a recently synthesized resin, or plastic, polyvinyl alcohol, known commercially as "PVA."1 This substance when combined with lactic acid and phenol provides a permanent hyaline mount and also clears and dehydrates the specimen in a satisfactory manner. The latter features are especially advantageous when it is desired to preserve for teaching purposes or for study collections such biologic forms as the scabies mite, ticks, fleas and lice or their ova. In the preservation of cutaneous fungi, the method is adaptable to material removed from ordinary cultures of fungi, actively growing slide cultures and material in the form of scrapings of skin for the purpose of direct microscopic study. Polyvinyl alcohol was first employed in biologic work by Lubkin and Carsten2 as a medium for the elimination of dehydration in the preparation of References 1. "PVA" (Grade RH-349), a synthetic polymer of vinyl alcohol, is available through the E. I. du Pont de Nemours Company, R. & H. Chemical Department, Niagara Falls, New York, at a cost of approximately $1 per pound. 2. Lubkin, V., and Carsten, M.: Elimination of Dehydration in Histological Technique , Science 95:2477 ( (June 19) ) 1942.Crossref 3. Downs, W. G.: Polyvinyl Alcohol: A Medium for Mounting and Clearing Biological Specimens , Science 97:2528 ( (June 11) ) 1943.Crossref 4. Dr. Ethel M. Rockwood made this suggestion.
TI - SIMPLE PLASTIC MOUNT FOR PERMANENT PRESERVATION OF FUNGI AND SMALL ARTHROPODS
JO - Archives of Dermatology and Syphilology
DO - 10.1001/archderm.1947.01520120051003
DA - 1947-12-01
UR - https://www.deepdyve.com/lp/american-medical-association/simple-plastic-mount-for-permanent-preservation-of-fungi-and-small-600B1LV0c0
SP - 763
EP - 765
VL - 56
IS - 6
DP - DeepDyve
ER -