TY - JOUR
AU - P. N. Styne and J. M. Vierling
AB -  for the study of hepatic metabolism,it has rarely been applied to the study of mice, This is primarily due to the lack of a simple,reproducible technique to cannulate the delicate portal vein initiate perfusion without irreversible hepatic injury. Therefore, we developeda technique to overcome these obstacles performed studiesto assess viability of the perfused the murine liver. The inflow catheter assemblypermits rapid, reproducible cannulation of the portal vein protects the cannulated vein from the effects of subsequentmanipulations. Serial assessments perfusion pressure,flow rate, perfusate of pH, oxygen consumption, fluxes of alanine aminotransferase potassium indicate that the murine liver remains viable during perfusion. Moreover, the light electron mi- logical perfusion of the murine liver in situ, This technique circumvents inherent difficulties in cannulating the murine portal vein establishing a physiological perfusion before hepatic ischemia occurs. METHODS G720 P. N. STYNE segment to minimize dilution of the injected substance. During laparotomy portal venous catheterization, the platform was held in a circuit-board clamp vise. Before beginning perfusion, the platform was moved to the perfusion box the vertical metal rod (Fig. 2) was secured in a holder. The pressure required to perfuse through each inflow catheter was determined before a cannulation. Generally, a pressure 
TI - Isolated perfusion of murine liver in situ: technique and validation
JF - AJP - Gastrointestinal and Liver Physiology
DA - 1985-12-01
UR - https://www.deepdyve.com/lp/the-american-physiological-society/isolated-perfusion-of-murine-liver-in-situ-technique-and-validation-5Rj3h2NX2A
VL - 249
IS - 
DP - DeepDyve
ER -