TY - JOUR
AU1 - Wang, Jia
AU2 - Liu, Zhiping
AU3 - Li, Ganqiong
AU4 - Li, Ji
AU5 - Kim, Hee-Joo
AU6 - Shelver, Weilin
AU7 - Li, Qing
AU8 - Xu, Ting
AB - Twenty-five phages that selectively bind to a monoclonal antibody (Mab) 1H2 specific to 2,2′,4,4′-tetrabromodiphenyl ether (BDE47) in the absence or presence of BDE47 have been selected from phage‐display libraries containing cyclic 7-mer, linear 7-mer, and linear 12-mer randomized peptides. Competitive and noncompetitive enzyme-linked immunosorbent assays (ELISA) for BDE47 were developed by using a clone C7-1 specific to the BDE47-free Mab 1H2 and a clone XC7-8 specific to the BDE47-bound Mab 1H2, respectively. The half-maximum signal inhibition concentration (IC50) of the competitive phage ELISA and the half-maximum signal enhancement concentration (EC50) of the noncompetitive phage ELISA for BDE47 were 6.8 ng mL−1 and 4.2 ng mL−1, respectively. The noncompetitive phage ELISA showed higher cross-reactivity with BDE28, BDE99, and BDE100 than the competitive one, ranging between 1.3 and 6.5 % versus 0.3 and 0.8 %. Recoveries of the competitive and the noncompetitive phage ELISAs for BDE47 in sewage sludge and fillet samples were 96–124 % and 97–120 %, respectively. The results of the two types of phage ELISAs for BDE47 in the real-world samples agreed well with a gas chromatography/electron capture detector-ion trap mass spectrometer method.
TI - Simultaneous development of both competitive and noncompetitive immunoassays for 2,2′,4,4′-tetrabromodiphenyl ether using phage-displayed peptides
JF - Analytical and Bioanalytical Chemistry
DO - 10.1007/s00216-013-7364-5
DA - 2013-10-06
UR - https://www.deepdyve.com/lp/springer-journals/simultaneous-development-of-both-competitive-and-noncompetitive-5RhIBZPa4t
SP - 9579
EP - 9583
VL - 405
IS - 29
DP - DeepDyve
ER -