TY - JOUR
AU1 - Xu, Bao-Jun
AU2 - Wang, Qi-Jun
AU3 - Jia, Xiao-Qin
AU4 - Sung, Chang-Keun
AB - Abstract The purpose of this study was to optimize the solid state cultivation ofMonascus ruber on sterile rice. A single-level-multiple-factor and a single-factor-multiple-level experimental design were employed to determine the optimal medium constituents and to optimize carbon and nitrogen source concentrations for lovastatin production. Simultaneous quantitative analyses of the β-hydroxyacid form and β-hydroxylactone for of lovastatin were performed by the high performance liquid chromatography (HPLC) method with a UV photodiode-array (PDA) detector. The total lovastatin yield (4≈6 mg/g, average of five repeats) was achieved by adding soybean powder, glycerol, sodium nitrate, and acetic acid at optimal composition of the medium increased by almost 2 times the yield observed prior to optimization. The experimental results also indicated that the β-hydroxylactone form of lovastatin (LFL) and the β-hydroxyacid form of lovastatin (AFL) simultaneously existed in solid state cultures ofMonascus ruber, while the latter was the dominant form in the middle-late stage of continued fermentation. These results indicate that optimized culture conditions can be used for industrial production of lovastatin to obtain high yields.
TI - Enhanced lovastatin production by solid state fermentation of Monascus ruber
JF - "Biotechnology and Bioprocess Engineering"
DO - 10.1007/BF02931187
DA - 2005-02-01
UR - https://www.deepdyve.com/lp/springer-journals/enhanced-lovastatin-production-by-solid-state-fermentation-of-monascus-5Q0Nn5VkK9
SP - 78
EP - 84
VL - 10
IS - 1
DP - DeepDyve
ER -