TY - JOUR
AU - Inoue,, Keizo
AB - Abstract Monoclonal antibodies were raised against rabbit platelet cytosolic arachidonoyl-preferential phospholipase A2. The antibodies precipitated the arachidonoyl-preferential phospholipase A2 activity in the soluble fraction of a rabbit platelet lysate in combination with an immobilized anti-mouse immunoglobulin antibody, and reacted predominantly with a protein exhibiting a molecular weight of approximately 88,000 on immunoblotting analysis. All three antibodies established so far reacted with human platelet arachidonoyl-preferential phospholipase A2 as effectively as the rabbit platelet enzyme. One of them reacted with the rat platelet arachidonoyl-preferential enzyme, whereas none of them reacted with rabbit platelet secretory 14-kDa group II phospholipase A2. The existence of an immunologically related phospholipase A2 was further shown in rabbit granulocytes, brain, lung, and liver, rat and mouse mast cells, and human monocytoma U937 cells. Thus, an arachidonoyl-preferential phospholipase A2 with similar structural properties appeared to be expressed in a variety of cells and tissues. This content is only available as a PDF. Author notes 1 This work was supported in part by Granta-in-Aid (Nos. 02557090, 03680163, and 63480490) for Scientific Research from the Ministry of Education, Science, and Culture of Japan. © BY THE JOURNAL OF BIOCHEMISTRY
TI - Immunochemical Detection of Arachidonoyl-Preferential Phospholipase A2
JO - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a123718
DA - 1992-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/immunochemical-detection-of-arachidonoyl-preferential-phospholipase-a2-4xBksy0Z2A
SP - 54
EP - 60
VL - 111
IS - 1
DP - DeepDyve
ER -