TY - JOUR
AU - BARDIN, C., WAYNE.
AB - Abstract Immunostainable inhibin a-subunit has been demonstrated in rat testes in a pattern consistent with localization in Sertoli cells. In the present study the distribution of asubunit immunostaining was compared to those of β-A- and β-B-subunits. Immunostaining of a-subunit was present in the seminiferous epithelium of fetal, neonatal, pubertal, and adult rats as well as in Sertoli cells in culture. The distribution of inhibin β-B-subunit immunostaining in this epithelium was consistent with Sertoli cell localization similar to that of the asubunit. The predominant staining with antibodies against the β-A-subunit was in nuclei of immature germ cells around the periphery of each seminiferous tubule. The most probable localization of this staining was in the nuclei of pachytene and zygotene spermatocytes. Specific immunostaining with β-A-subunit antiserum was also evident in the seminiferous epithelium adjacent to the tubular lumen. Immunoreactive a- and β-Asubunit staining was present in a Leydig cell line, and β-A immunoreactivity was present in interstitial cells of neonatal rat testes. After hypophysectomy, inhibin a-subunit immunostaining decreased, β-A-subunit staining did not change, and β-B-subunit staining increased. We conclude that immunoreactive inhibin subunits are present in multiple cells in the testis and that the amounts of immunostainable subunits in the seminiferous epithelium are differentially regulated This content is only available as a PDF. Author notes * This work was supported by the Clayton Foundation; NIH Grants HD-16149, HD-13527, DK-34449, HD-13541, HD-12357, and DK- 26741; and a cooperative agreement (DPE-3005-A-00-3003-00) and Grant pha-GH6 from the U.S. Agency for International Development. † Supported by the Biotechnology Career Fellowship Program of the Rockefeller Foundation. Copyright © 1989 by The Endocrine Society
TI - Immunostainable Inhibin Subunits Are in Multiple Types of Testicular Cells
JO - Endocrinology
DO - 10.1210/endo-125-4-1941
DA - 1989-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/immunostainable-inhibin-subunits-are-in-multiple-types-of-testicular-4d0aM3KE7M
SP - 1941
EP - 1950
VL - 125
IS - 4
DP - DeepDyve
ER -