TY - JOUR
AU - Bueno, Alejandro
AB - LETTERS To the Editor: Talks at the 2013 American Contact Dermatitis Meeting emphasized the need for better standardization of patch testing 1,2 and use of stable, reliable patch test allergens. Commercial allergens are supplied in containers, which do not allow the exact measure of amounts dispensed. Because perfect standardization is unattainable for busy patch test centers using currently supplied allergen dispensers, we developed reasonable methods that are closer to the ideal standardization. Regarding petrolatum allergens, 20 mg has been recommended as the ideal for Finn chambers. Staff training was performed using empty Chemotechnique syringes (Vellinge, Sweden) filled with white petrolatum and green food color. An ideal Chemotechnique IQ Ultra strip of 10 allergens containing 200 mg of colored pet- rolatum (calculated by weighing the filled strip and subtracting the weight of the empty strip) was prepared as a model. This strip was pressed against clear plexiglass so that staff could see that the strip filled, but did not overfill, the chambers. A second strip was prepared with too much or too little allergen and pressed against FIGURE 1. Top strip: overfilled and underfilled chambers pressed clear plexiglass so that the trainee could see the chambers that were against Plexiglass. 
TI - Practical Standardization of Patch Testing
JF - Dermatitis
DO - 10.1097/DER.0000000000000021
DA - 2014-03-01
UR - https://www.deepdyve.com/lp/wolters-kluwer-health/practical-standardization-of-patch-testing-4Qo0tqEiLm
SP - 96–97-96&ndash
EP - ndash;97-96–97
VL - 25
IS - 2
DP - DeepDyve
ER -