TY - JOUR
AU - Laue, Michael
AB - Diagnostic electron microscopy is a useful technique for the identification of viruses associated with human, animal, or plant diseases. The size of virus structures requires a high optical resolution (i.e., about 1 nm), which, for a long time, was only provided by transmission electron microscopes operated at 60 kV and above. During the last decade, low-voltage electron microscopy has been improved and potentially provides an alternative to the use of high-voltage electron microscopy for diagnostic electron microscopy of viruses. Therefore, we have compared the imaging capabilities of three low-voltage electron microscopes, a scanning electron microscope equipped with a scanning transmission detector and two low-voltage transmission electron microscopes, operated at 25 kV, with the imaging capabilities of a high-voltage transmission electron microscope using different viruses in samples prepared by negative staining and ultrathin sectioning. All of the microscopes provided sufficient optical resolution for a recognition of the viruses tested. In ultrathin sections, ultrastructural details of virus genesis could be revealed. Speed of imaging was fast enough to allow rapid screening of diagnostic samples at a reasonable throughput. In summary, the results suggest that low-voltage microscopes are a suitable alternative to high-voltage transmission electron microscopes for diagnostic electron microscopy of viruses.
TI - Diagnostic Electron Microscopy of Viruses With Low-voltage Electron Microscopes
JO - Journal of Histochemistry & Cytochemistry
DO - 10.1369/0022155420929438
DA - 2020-06-01
UR - https://www.deepdyve.com/lp/sage/diagnostic-electron-microscopy-of-viruses-with-low-voltage-electron-47HOCdcFul
SP - 389
EP - 402
VL - 68
IS - 6
DP - DeepDyve
ER -