TY - JOUR
AU - Dunning, Mary
AB - A NE\V METHOD OF STAINING ACID-FAST BACTERIA AND SPORES V I C TOR BUR K E AND MAR Y DUN N I N G From the Bacteriological Laboratory, State College of Washington, Pull man A review of the numerous methods of staining the tubercle bacilli discloses that each of the steps in the staining has been subjected to numerous modifications. Which of the methods, if any, give results superior to the Ziehl- N ee1son method remains to be determined. Cer­ tain of the modifications, however, while possibly not giving superior results, may have certain advantages as regards convenience, time and cheapness. The present paper describes the use of acetone and iodine acetone as the decolorizer in the differential staining of tubercle bacilli and of bacterial spores. Notes on other factors affecting the staining of tubercle bacilli are included. METHOD OF STAINING TUBERCLE BACILLI 1. Place slide in a beaker of steaming carbol-fuchsin (1.S gm. basic fuchsin, 15 c c. of 95% alcohol, 5 c c. carbolic acid crystals, 8S c c. distilled water) for 2 minutes or longer. 2. Wash without allowing smear to become dry. 3. Decolorize with acetone or iodine acetone (l part Gram's iodine solution to 
TI - New method of staining acid-fast bacteria and spores
JO - The Journal of Infectious Diseases
DO - 10.1093/infdis/34.2.105
DA - 1924-02-01
UR - https://www.deepdyve.com/lp/oxford-university-press/new-method-of-staining-acid-fast-bacteria-and-spores-40lsQX0uKM
SP - 105
EP - 109
VL - 34
IS - 2
DP - DeepDyve
ER -