TY - JOUR
AU - Jones, Huw D.
AB - mainly due to the clear structure with plenty of scientific markers to localise translational fusion proteins, and RNAi to detail. The careful selection of literature references for each silence pre-defined maize genes are particularly interesting chapter makes the volume very effective and a must-read for scientists using transgenic approaches in their research. for specialists and novices in addition to maize researchers The four chapters on transformation methods compare biolis- and breeders as well. tics, Agrobacterium tumefaciens and silicon carbide whiskers for DNA-delivery into immature zygotic embryos or compe- tent callus cultures derived from HiII, H99 and other regen- Ralf G. Kynast erable genotypes. They also provide detailed instructions for E-mail: r.kynast@kew.org making and sterilising the various tissue culture media required and the commonly used selection systems based on resistance to specific antibiotics or herbicides. The detailed methods and additional notes make the transform- ation of maize accessible to anyone with a reasonably equipped laboratory and plant growth facilities. I particularly doi:10.1093/aob/mcs078 like the note in the chapter on DNA hybridisation that sug- Published electronically: 12 April 2012 gests ‘an unwanted book is ideal for weighing down the blot- ting stack’ and immediately pictured the author’s stained Transgenic maize: methods 
TI - Transgenic maize: methods and protocols
JO - Annals of Botany
DO - 10.1093/aob/mcs078
DA - 2012-06-12
UR - https://www.deepdyve.com/lp/oxford-university-press/transgenic-maize-methods-and-protocols-40Zyf2runW
SP - ix
EP - ix
VL - 109
IS - 7
DP - DeepDyve
ER -