TY - JOUR
AU1 - Michelot, Alphee
AU2 - 
AB - RESEARCH ARTICLE Synergy between Wsp1 and Dip1 may initiate assembly of endocytic actin networks 1 2 1 1† Connor J Balzer , Michael L James , Heidy Y Narvaez-Ortiz , Luke A Helgeson , 2 1 Vladimir Sirotkin , Brad J Nolen * Department of Chemistry and Biochemistry, Institute of Molecular Biology, University of Oregon, Eugene, United States; Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, United States Abstract The actin filament nucleator Arp2/3 complex is activated at cortical sites in Schizosaccharomyces pombe to assemble branched actin networks that drive endocytosis. Arp2/3 complex activators Wsp1 and Dip1 are required for proper actin assembly at endocytic sites, but how they coordinately control Arp2/3-mediated actin assembly is unknown. Alone, Dip1 activates Arp2/3 complex without preexisting actin filaments to nucleate ‘seed’ filaments that activate Wsp1- bound Arp2/3 complex, thereby initiating branched actin network assembly. In contrast, because Wsp1 requires preexisting filaments to activate, it has been assumed to function exclusively in propagating actin networks by stimulating branching from preexisting filaments. Here we show that Wsp1 is important not only for propagation but also for initiation of endocytic actin networks. Using single molecule total internal reflection fluorescence microscopy we show 
TI - Decision letter: Synergy between Wsp1 and Dip1 may initiate assembly of endocytic actin networks
DO - 10.7554/elife.60419.sa1
DA - 2020-07-24
UR - https://www.deepdyve.com/lp/unpaywall/decision-letter-synergy-between-wsp1-and-dip1-may-initiate-assembly-of-3sdzLn8kf2
DP - DeepDyve
ER -