TY - JOUR
AU - Chang, Woo‐Hyun
AB - The crystalline surface layer (S‐layer) protein (SLP) of Rickettsia typhi is known as the protective antigen against murine typhus. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32‐kDa protein. To express only the SLP from this gene, the putative signal sequence and the 32‐kDa protein portion were removed from the slpT. This protein was expressed in Escherichia coli as a fusion protein, consisting of the SLP and maltose binding protein. The recombinant protein reacted strongly with polyclonal antiserum of a patient with murine typhus.
TI - Expression and Purification of the Crystalline Surface Layer Protein of Rickettsia typhi
JO - Microbiology and Immunology
DO - 10.1111/j.1348-0421.1996.tb03339.x
DA - 1996-01-01
UR - https://www.deepdyve.com/lp/wiley/expression-and-purification-of-the-crystalline-surface-layer-protein-3iY12dWsOP
SP - 233
EP - 236
VL - 40
IS - 3
DP - DeepDyve
ER -