TY - JOUR
AU - Widmalm, Göran
AB - The tetrasaccharide lacto‐N‐neotetraose β‐D‐Galp‐(1→4)‐β‐D‐GlcpNAc‐(1→3)‐β‐D‐Galp‐(1→4)‐D‐Glcp was dissolved in a dilute mixture of dimyristoyl‐ and dihexanoylphosphatidylcholine in deuterium oxide and 1J(C,H) spin–spin coupling constants were measured in an isotropic solution at 25°C. On raising the temperature to 38°C, an ordering of the system occurs which permits determination of dipolar couplings. An energy mini‐mized molecular mechanics model was generated showing that the molecule is anisotropic and can be treated as a cylinder. A fit of the expression for the carbon–proton dipolar couplings in a uniaxial molecule in a uniaxial liquid crystal revealed good agreement with experimental data, emphasizing the future potential of the method in studies of carbohydrate conformation. © 1998 John Wiley & Sons, Ltd.
TI - NMR investigation of oligosaccharide conformation using dipolar couplings in an aqueous dilute liquid crystalline medium
JO - Magnetic Resonance in Chemistry
DO - 10.1002/(SICI)1097-458X(1998100)36:10<773::AID-OMR399>3.0.CO;2-4
DA - 1998-10-01
UR - https://www.deepdyve.com/lp/wiley/nmr-investigation-of-oligosaccharide-conformation-using-dipolar-3P7Shv4qDL
SP - 773
EP - 776
VL - 36
IS - 10
DP - DeepDyve
ER -