TY - JOUR
AU1 - , Van Blerk, M.
AU2 - Nijs,, M.
AU3 - , Van Steirteghem, A. C.
AB - Abstract The present study reports a biopsy technique on decompacted late mouse morulae. Zygotes were collected from hyperstimulated F1 hybrids (C57BL6 females × CBAcamales) and cultured in modified Earle's balanced salt solution supplemented with 0.5% bovine serum albumin until the late morula stage (92 h post administration of human chorionic gonadotrophin). Decompaction was obtained by exposure of the embryos to Ca2+-Mg2+-free phosphate-buffered saline (PBS) or an aqueous solution of ethylene-diaminetetraacetic acid (EDTA) and glycine. Using micro-manipulation, a single blastomere was aspirated without removal or softening of the zona. The impact of the different decompaction procedures and the biopsy technique was studied by vital staining, by in-vitro culture up to the early egg-cylinder staged and by the recovery of living mice after transfer to pseudopregnant foster mothers. Our investigations revealed no impact of decompaction and biopsy on immediate viability, as assessed by fluorescein diacetate staining. A significant reduction (P < 0.05) in the number of mouse morulae that reached the early egg-cylinder stage in vitro was observed after the biopsy procedure. We observed that after this microbiopsy technique, successful pregnancies can be obtained but at a lower percentage compared to controls (P < 0.01). decompaction, embryo viability, late mouse morulae, micromanipulation, preimplantation diagnosis This content is only available as a PDF. © Oxford University Press
TI - Decompaction and biopsy of late mouse morulae: assessment of in-vitro and in-vivo developmental potential
JF - Human Reproduction
DO - 10.1093/oxfordjournals.humrep.a137531
DA - 1991-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/decompaction-and-biopsy-of-late-mouse-morulae-assessment-of-in-vitro-2oy103sdtB
SP - 1298
EP - 1304
VL - 6
IS - 9
DP - DeepDyve
ER -