TY - JOUR
AU1 - Simon,, Carlos
AU2 - Frances,, Ana
AU3 - Lee, Bo, Yon
AU4 - Mercader,, Amparo
AU5 - Huynh,, Tri
AU6 - Remohi,, Jose
AU7 - Polan, Mary, Lake
AU8 - Pellicer,, Antonio
AB - Abstract There is evidence suggesting the importance of the interleukin-1 receptor type I (IL-1Rtl) and interleukin-1β(lL-1β]as mediators in local intercellular interactions in endometrial tissue and embryonic implantation. To complete our understanding of the entire endometrial IL-1 system in humans, we have investigated the immunohistochemical distribution of IL-1 receptor antagonist (IL-1ra) in the human endometrium throughout the menstrual cycle. We have also identified the forms of IL-1ra present in human endometrial cells. Immunoreactive IL-1ra was found in both cryostat and paraffin-embedded sections of human endometrium using the alkaline phosphatase-peroxidase (A-P) method with two different IL-1ra antibodies. IL-1ra was present throughout the entire menstrual cycle, located primarily in the endometrial epithelium. However, IL-1ra staining was significantly higher during follicular phase in comparison with early and mid-late luteal phases. Reverse transcriptase polymerase chain reaction of cultured stromal and glandular cells showed that these cells express the intracellular form of IL-1ra mRNA (iclL-1ra). Our results demonstrate the regulated presence of the iclL-1ra in the human endometrium. This finding supports a possible autocrine-paracrine role for the IL-1 system in the human endometrium and embryonic implantation. human. endometrium, interleukin-1 receptor antagonist, localization, reverse transcriptase polymerase chain reaction This content is only available as a PDF. Author notes *Presented in part at the 50th Annual Meeting of the American Fertility Society, San Antonio, Texas, November 5–10, 1994. © Oxford University Press
TI - Endocrinology and paracrinology: Immunohistochemical localization, identification and regulation of the interleukin-1 receptor antagonist in the human endometrium
JF - Human Reproduction
DO - 10.1093/oxfordjournals.humrep.a136326
DA - 1995-09-01
UR - https://www.deepdyve.com/lp/oxford-university-press/endocrinology-and-paracrinology-immunohistochemical-localization-2eum0thU9Z
SP - 2472
EP - 2477
VL - 10
IS - 9
DP - DeepDyve
ER -