TY - JOUR
AU1 - Rothstein, Nathaniel
AU2 - Wolman, Flora
AB - STUDIES OF THE IMMUNOCHEMISTRY OF LEPTOSPIRES III. SEROLOGIC CHARACTERIZATION OF THE COMPLEMENT-FIXATION ANTIGEN NATHANIEL J{OTHSTEIN AND FLORA WOLMAN From the Division of Veterinary Medicine, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington 12, D. C. has been referred to by McComb et al Extraction of leptospiral cells with (1957) as the sensitized erythrocyte ly­ 70% ethanol yielded serologically reac­ sis (SEL) test. A study of the immuno­ tive clear preparations which became genicity of these leptospiral extracts is opalescent when the alcohol was re­ still in progress. Preliminary data avail­ moved by dialysis. This opalescent ex­ able indicate that the CF antigen is tract was separated into a water-soluble capable of eliciting homologous and and a water-insoluble phase by electro­ limited heterologous protection. This dialysis followed by centrifugation. The communication presents the results of a water-soluble fraction has been de­ study of the stability of these prepara­ scribed in previous reports by Rothstein tions and the reactivity of the CF anti­ and Hiatt (1956), and Rothstein (1957), gens in the HL and CF 52 technics. In who referred to it as the precipitinogen. addition, this report will briefly describe The water-insoluble phase, referred to the 
TI - Studies of the Immunochemistry of Leptospires III. Serologic Characterization of the Complement-Fixation Antigen
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/105.3.280
DA - 1959-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/studies-of-the-immunochemistry-of-leptospires-iii-serologic-1j7A54xpFG
SP - 280
EP - 287
VL - 105
IS - 3
DP - DeepDyve
ER -