TY - JOUR AU1 - Holman, W. L. AB - THE RELATIVE LONGEVITY OF DIFFERENT STREPTO­ COCCI AND POSSIBLE ERRORS IN THE ISOLA­ TION AND DIFFERENTIATION OF STREPTOCOCCI * W. L. HOLMAN (From the Pathological Laboratories, University of Pittsburgh, Pittsburgh, Pa.) In the study of a large number of strains of streptococci I am impressed by the marked variation in the viability of the different strains. I have found that many types of streptococci may be stored for months on various media, while on the other hand, one frequently encounters strains that require fresh food material at short intervals. The most favorable media for the preservation of the life of streptococci are those in which the organisms do not readily produce self-destructive substances. Such media are plain serum broth, blood agar, gelatin and others. It is very important that the media do not contain available carbohydrates as the acids formed by fermentation "are detrimental to the life of these organisms. EFFECTS OF ACID Numerous investigators have observed the destructive effect of acids on streptococci. Koch and Pokschischewsky' used the fact that the streptococcus equi remained living in mannite serum broth after ten days as evidence that it had not attacked the carbohydrate. They also found that, by neutralizing at TI - The Relative Longevity of Different Streptococci and Possible Errors in the Isolation and Differentiation of Streptococci JF - The Journal of Infectious Diseases DO - 10.1093/infdis/15.2.293 DA - 1914-09-01 UR - https://www.deepdyve.com/lp/oxford-university-press/the-relative-longevity-of-different-streptococci-and-possible-errors-1a63OqhfZd SP - 293 EP - 308 VL - 15 IS - 2 DP - DeepDyve ER -