TY - JOUR
AU - Eckard, R. J.
AB - Abstract Enteric methane produced by ruminants is a source of greenhouse gas emissions. One method for investigating methane production from ruminants is the in vitro method which when compared with in vivo methods is faster and less expensive. The ANKOMTM system is an in vitro system that periodically releases excess gas during the incubation to prevent it from diffusing into the medium. For this reason, a gas sample taken from the module's headspace at the conclusion of the incubation period may not be representative of the gas produced during the entire fermentation period. This study tested two methods that enable the collection of released gases. Yeast and sugar were incubated for 24 h in 310 mL ANKOMTM bottles equipped with an ANKOM module to regulate headspace pressure through ventilation. Incubations were made with three different methods: vented gas not collected (NC); vented gas collected in gas bags through a 304 cm gas sample line with an internal diameter (ID) of 1.0 mm (C304); and vented gas collected in gas bags through a 22 cm extension tube with an ID of 4.0 mm (C22). Each method was conducted using four different venting pressures (0.4, 0.6, 0.8, and 1.0 psi). When total gas production was calculated from absolute pressure measurements made by the pressure transducer in the ANKOM module, the mean of total gas production (ml) for the C304 method was significantly (P < 0.05) greater (125.3 ± 1.9) than either the C22 method (114 ± 1.9) or the NC method (115 ± 1.7), while the C22 method was not different from the NC method. There was no effect of venting pressure across treatments on estimated total gas production. It is concluded that the C22 method for collecting gas can be used in gas production studies with the ANKOM system as it does not interfere with measurement of gas production. American Society of Animal Science
TI - 1188 A novel method for collecting gas produced from the in vitro ANKOM gas production system
JF - Journal of Animal Science
DO - 10.2527/jam2016-1188
DA - 2016-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/1188-a-novel-method-for-collecting-gas-produced-from-the-in-vitro-1TSO8UbcCT
SP - 570
EP - 570
VL - 94
IS - suppl_5
DP - DeepDyve
ER -