TY - JOUR
AU1 - P. Bodin , C. Travo , J. C. Stoclet , and P. Travo
AB -  MATERIALS AND METHODS Animals Thirteen-week-old male WKY and SHR originating from the Okamoto strain (17) were used. Animals were bred in the sterile animal house of the laboratory from pairs supplied by the Centre de Selection et dElevage dAnimaux de Laboratoire, Orleans, France. These animals were exposed to the same standard light-dark cycles and had the same standard diet. The blood pressure was measured by plethysmography on conscious animals just before use. Rats were killed by stunning followed by cervical dislocation. Cell Suspensions Cell suspensions were prepared as previously described (4,6). Briefly, three rat aortas were used to initiate a primary culture. The vessels were dissected and placed in complete culture medium (CCM). This culture medium was comprised of minimum essential medium and Hams F-10 medium with Earles salts (19 vol/vol; Eurobio, Paris, France) supplemented with 2 mM glutamine, 100 U/ml penicillin G, 0.05 mM vitamin C, and 2% serum substitute Ultroser G/Ultroser SF (1:3 vol/vol; IBF, Villeneuve-la-Garenne, France). Aortas were treated with collagenase (CLS II; Worthington, Freehold, NJ), 70 U/ml in Hanks balanced salt solution for 30 min at 37OC. The adventitias were then mechanically stripped, and the intimas were removed using a fine paintbrush. The resulting 
TI - High sensitivity of hypertensive aortic myocytes to norepinephrine and angiotensin
JF - AJP - Cell Physiology
DA - 1993-02-01
UR - https://www.deepdyve.com/lp/the-american-physiological-society/high-sensitivity-of-hypertensive-aortic-myocytes-to-norepinephrine-and-1QvSMpsyZv
VL - 264
IS - 
DP - DeepDyve
ER -