TY - JOUR
AU - Zhong, Hongbo
AB - A new method using MCE with LIF detection was developed for the determination of hydrogen peroxide (H2O2). Bis(p‐methylbenzenesulfonyl)dichlorofluorescein, a new fluorogenic reagent synthesized by our laboratory was employed as a labeling reagent, the derivatization reaction was performed in 0.10 M HEPES buffer (pH 7.4) for 30 min at 37°C. The detection of H2O2 was accomplished in 55 s, using a 40 mM HEPES buffer, 20% mannitol, pH 7.4, on a glass microchip. The RSDs of migration time and peak area were 1.8 and 3.7%, respectively. Method validation showed the linear response ranging from 0.50 to 50 μM with an LOD (S/N=3) of 0.20 μM (19.1 amol). The proposed method was applied to determine H2O2 in phorbol myristate acetate‐stimulated RAW264.7 macrophages, the concentration of H2O2 was found to be 1.86±0.05 μM; recoveries for macrophage samples were from 96.7 to 97.8%, within‐days and between‐days accuracies were 4.5% (n=5) and 6.8% (n=5), respectively.
TI - A new route for simple and rapid determination of hydrogen peroxide in RAW264.7 macrophages by microchip electrophoresis
JF - Electrophoresis
DO - 10.1002/elps.200800635
DA - 2009-06-01
UR - https://www.deepdyve.com/lp/wiley/a-new-route-for-simple-and-rapid-determination-of-hydrogen-peroxide-in-1QmUsjnjYQ
SP - 1983
EP - 1990
VL - 30
IS - 11
DP - DeepDyve
ER -