TY - JOUR
AU1 - Escriva, Anthony
AB - Brain inulin space in developing kittens using ventriculocisternal perfusion Bennett A. Shaywitz,* M.D., and Anthony Escriva (technical assistance) THE EXTRACELLULAR SPACE (ECS) of the brain carried out in 11 kittens and 5 adult cats. The has been difficult to define, with estimates perfusate consisted of simulated cerebrospinal varying considerably depending on the methods fluid containing appropriate salt concentrations (Table 1) .l-18 Parenteral administration as recommended by MerkZ0 After addition used of inulin-I4COOH, 2.5 pc. per milliliter, the of tracer amounts of chemical indicators are pH of the perfusate was adjusted to 7.35 with widely used to measure the extracellular space of many tissues. This method, when applied to CO,. brain ECS, yields relatively low values in ma- Inflow rates were determined with a Har- ture animals. However, this may be an under- vard perfusion apparatus with a precalibrated syringe. The volume of the fluid perfused estimate due to limited transport of the indi- through the system was maintained at a con- cators across the blood-brain barrier. stant rate in a given experiment. The rate of In an effort to circumvent the blood-brain perfusion for both adult cats and 1- to 2- barrier, Rall et al.13 and Katzman et aL14 
TI - Brain inulin space in developing kittens using ventriculocisternal perfusion
JF - Neurology
DA - 1972-03-01
UR - https://www.deepdyve.com/lp/wolters-kluwer-health/brain-inulin-space-in-developing-kittens-using-ventriculocisternal-1MQi3uNwWd
SP - 238–245-238&ndash
EP - ndash;245-238–245
VL - 22
IS - 3
DP - DeepDyve
ER -