TY - JOUR
AU - Hauser, Conrad
AB - We studied the involvement of chemokines that bind to G protein‐coupled receptors in the migration of skin homing T cells across a bilayer vascular construct (BVC) consisting of a fibroblast matrix underneath an activated endothelial (EC) monolayer. Based on the expression of the cutaneous lymphocyte‐associated antigen (CLA), a skin homing receptor, CD45R0+ T cells freshly isolated from blood or HUT‐78 cutaneous T lymphoma cells were separated into CLA+ and CLA− subpopulations. These T cells were incubated on interleukin (IL)‐1β and tumor necrosis factor‐α‐activated EC, and the number of transmigrated cells was determined. The chemokine IL‐8 was selectively involved in the enhanced migration of CLA+ T cells across activated EC as demonstrated by blocking antibody to IL‐8 but not to GRO‐α, MCP‐1 and RANTES. Identical results were obtained with both human umbilical vein EC (HUVEC) and microvascular skin EC (HDMEC). Pertussis toxin selectively inhibited the enhanced transendothelial migration (TEM) of CLA+ T cells, suggesting that CLA‐dependent TEM depends on Gi protein‐transmitted signals. Moreover, the IL‐8 receptor B (IL‐8RB) appeared to be functionally involved in TEM, as demonstrated by receptor desensitization with the CXC chemokines IL‐8 and GRO‐α and by blocking the IL‐8RB with specific monoclonal antibodies. Although only the IL‐8RB was involved in CLA‐dependent TEM, mRNA encoding IL‐8RA and IL‐8RB was expressed by both CLA+ and CLA− T cells. This correlated with IL‐8RA and IL‐8RB surface expression on these cells. Thus, the IL‐8RB is selectively functional in TEM of T cells expressing the skin homing receptor CLA. Our results demonstrate a critical role for IL‐8 and possibly other IL‐8RB ligands in addition to the IL‐8RB in TEM and suggest the involvement of these molecules in the homing of specific T cells to inflamed skin.
TI - The interleukin‐8 receptor B and CXC chemokines can mediate transendothelial migration of human skin homing T cells
JO - European Journal of Immunology
DO - 10.1002/eji.1830260914
DA - 1996-09-01
UR - https://www.deepdyve.com/lp/wiley/the-interleukin-8-receptor-b-and-cxc-chemokines-can-mediate-1LL0dequs7
SP - 2056
EP - 2061
VL - 26
IS - 9
DP - DeepDyve
ER -